有機磷降解菌的構建與微生物肥料的研究.pdf

1、浙江大學博士后學位論文有機磷降解菌的構建與微生物肥料的研究姓名：胡秀芳申請學位級別：博士后專業(yè)：微生物學指導教師：陳集雙20030701charactericsofbothionsandbacteriaSporeswerelesssensitivethannormalbacteria，andcapsuleprotectsporesfromseveredamageIonsenhancedlethalratewithincreasingof

2、ions，butnodifferenteffectwerefoundbetweenionswithvariousspeciesandenergyThelethalrateof曠with101510拍ions／cm2onKNP414were60％～95％Themutant021120degrade25％phytateacidinNBRIPmediumandthedegradationrateincreasedto475％inrevised

3、NBRIPmediumTheconstitutionofrevisedmediumwere：glucose10％，(NH4)2S04O2％，sodiumphytate02％，KClO05％，MgS04’7H20005％，MnS04‘H200001％andFeS047H200001％，CaC0301％，pH70。021112wasanotherphytateaciddegradationbacteriaisolatedfromsoilBa

4、sedonphenotypiccharacteristicsandphylogeneticanalysis，021112wasidentifiedasEnterobactersakazakiiwith972％identity021112obviouslydegradephytateacidandlecithinwiththedegradationrateof8709％and8740％respectively021112alsodegra

5、dedphytateacidinrapeseedmealandswinemanureto2604％and1538％respectivelyHighalkalinephosphatasewasfoundinculturemedium，whichindicatedthepossibleactionofphosphataseindegradationoforganophosphateHoweverthecharacteristicsofpho

6、sphatasewereinfurtherstudy2耵leresearchofmicrobialfertilizerAshigheffectivepotassiumandphosphatedissolutionsilicatebacteriatheCultureTechnologyofKNP414wasstudiedAstotheresultsofsinglefactorexperimentstheoptimumculturewas3

7、％starchO2％yeast01％phosphateandO1％MgS04Orthogonalexperimentindicatedthattheoptimumcombinationwas1％starch02％Yeast01％Na2HP4and01％MgS04TheconditionsofculturewerealsoinvestigatedinflaskTheoptimumconditionsin500mlflaskwereasfo

8、llows：50mlmedium，02％CaC03，510％inoculum，250rpm／minrotatespeedUndertheoptimalmediumandfermentationconditions舯414wasculturedin70Lfermentorandtheproductwascertifiedtoberegularbyrelateddepartmentofgovernment3Theeffectofmicrob