海水池塘養(yǎng)殖凈水芽孢桿菌的篩選、生物學特性及其應用研究.pdf

1、河北農(nóng)業(yè)大學碩士學位論文海水池塘養(yǎng)殖凈水芽孢桿菌的篩選、生物學特性及其應用研究姓名：高海英申請學位級別：碩士專業(yè)：發(fā)酵工程指導教師：王占武20080601IsolationandStudyonBiologicalCharacteristicsofWaterPurificationBacillusandApplicationinMarineCulturePondAuthor：GAOHaiyingSupervisor：WANGZattanw

2、uMajor：FermentationengineeringAbstract11圮accumulationoforganicpollutants，ammonianitrogenandnitritenitrogenisthemaininducementfactorofaquaculturewaterdeteriorationdiseasesoccurrenceandqualitydecreasing、析tllfishandshrimpsT

3、heimplementationofbioremediationbybeneficialmicroorganismhasbecometheresearchanddevelopmentfocusatpresentToscreenandgaindominantBacillusstrainsortheircombinationswhichhaveexcellentwaterpurificationeffectsandpreparationpe

4、rformanceisthecruxtoformulateefficientmicroecologyWaterPurifyingProducts228BacillusstrainswereisolatedandpurifiedfromthemarinepondsedimentTwodominantstrains，BacillusT301andT905，wereobtainedthroughthesalttolerance，degrada

5、tionoforganicmatterandanamoniaandnitritenitrogenscreeningtestsnefourdaysammoniadegradationrateofT301Was5518％and5200％andnitritenitrogendegradationrateWas5595％and5152％separatelywhile，ammoniadegradationrateofT905WaS3525％and

6、3653％andthenitritenitrogendegradationrateWaS721O％and7417％separatelywhentheinitialammoniaandnitratenitrogenconcentrationWas20mg／Land40mg／Linsimulated療eshandseaaquaculturewaterBasedonmorphologicalfeaturesandresultsofphysio

7、logicalandbiochemicalcharacteristics，T301andT905WaSidentified嬲BacillussubtilisaccordingtoTheManualofSystematicMethodsofDeterminativeBacterialandBergeysManualofDeterminativeBacteriology硼kresultsofsafetytestsshowedthatthet

8、oxicityofthetwostrainsWaSactuallynontoxiconmiceandwhite1egshrimps1f11estudyofthebiologicalcharacteristicsofstrainsshowedthattheT301andT905strainscoulduseglucose。starchlactose，mannitol，sugarandothercarbonSOurceSn坨organicn

9、itrogenSOurceSofpeptone，yeastextractbeefGaodengweremoreconduciveforthegrowthofthebacteriathaninorganicnitrogenSOurce111eresultsofttestsshowedthatK2HP04andKH2P04hadsignificanteffects011thegrowthofBacillussubtilisT301andT9

10、05口005)皿把suitableconditionsfor1r301andT905growthwereconsistentwithwaterpurificationmappropriatetemperaturerangedfrom22“Cto38℃andpHvaluefrom6t085111einfluenceofsaltconcentrationWaSnotsignificantfortheirgrowthandwaterpurif