甘草酸ELISA法建立及栽培甘草產(chǎn)量與品質調控機理研究.pdf_第1頁
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1、Y773448分類號密級≮目差≮犬等博士學位論文單位代碼：10019學號：B02025甘草酸ELISA法建立及栽培甘草產(chǎn)量與品質調控機理研究StudyonELISAforGIycyrrhjzinicAcidandRegulationoftheYieldandQualityofLicorice(GlycyrrhizauralensisFisch)研究生：奎劇指導敦師：衄鮭盟熬援合作指導教師：至握民副煎攫申請學位專業(yè)研究所在j類級別：名稱：

2、方向：學院：壅堂盟掛翅栽董皇越佳堂魚疸筮塹皇塑旦揎物籃盅翊撞盔堂皇圭塑基薟主瞳一零零五年六月AbstractTheenzyme—linkedimmunosorbentassay(ELISA)forglycyrrhizinicacidinlicorice(GlycyrrhizauralensisFisch)wasdevelopedThemethoddevelopedwasusedforevaluatingthequalityoflicor

3、icetreatedbyplantgrowthsubstanceHKL4TheeffectsofHKL4oilyield，component，leafphysiologicalactivities，leafphotosynthesisandrootphysiologicalfunctioninlicoricewasalsoinvestigated1Itisthefirsttimethathaptensandglycyrrhizinica

4、cid(GA)werecovalentlyconjugatedtobovineserllmalbumin(BSA)orovalbumin(OVA)toprepareantigens(immanogens)andcoatingantigensbycarbodiimide(CDl)methodTheconjugateswereidentifiedandtheirmolecularratioofhaptentocarrierprotenwer

5、edeterminedbyUVspectrophotometryConjugatesGABSAwereusedtoimmunizeBalb／cmousetoprepawpolycloneantibodies(PcAbs)furGAThetitersofantiserawerehigherthan3210。Powderedlicoricerootsampleswe『eextractedThecontentsgiycyrrhizinicac

6、iddeterminedbyELISAagreedwellwiththosedeterminedbyHPLCTheELISAresultsweregoodcorrelationwithHPLCthecorrelationcoefficientis，=0991811leestablishmentoftheenzymelinkedimmunosorbentassay(ELlSA)forgiycyrrhizinieacidisreported

7、firsttimeinChina2ThecontentsofgiycyrrhizinicacidinlicoricerootswemdeterminedbyELISAItisthecooperationaltimeofthecontentsofglycyrrhizinieacidandyieldinthelaterautumnThecontentsofglycyrrhizinicacidtendedtobehigherintheyoun

8、gerpartsoftherootandintheolderpartsoftherhizome3HKL4regulatedthehormonalsystems，thenimprovedthecooperationalexpressionofyieldandqualityofrootsandrhizomesTheyieldofrootsandrhizomesincreasedby138％throughHKL4treatmentThecon

9、tentsofglycyrrhizinicacidandflavomesinrootsincreasedby324％and353475％respectivelyUsingofthefingerprintsofextractedionchromatograms(EIc)，thesafetyofchemicalcomponentsinthelicoricerootstreatedbyHKL4wasevaluatedNonewcompound

10、swasfound4BiochemcialcontrolincreasedthephysiologicalactivitiesinleavesHKL4treatmentssignificantlyincreasedtheactivityofSODandCATwhiletheactivityofPODandthecontentofMDAwasreducedTreatedbyHKL4，thesubmicroscopicstructureof

11、leafchangedChloroplastwasmostsensitive，thenmitochondriaWesupposedthattheappropriatedosageofplantgrowthsubstancecanimprovetheabilityofbiosynthesisandenergymetabolisminleafsothatphotochemicalefficieneies(Fv／Fm)increased5Th

12、ephysiologicalactivityofrootwasimprovedbyHKL4treatmentHK卜4treatmentssignificantlyincreasedtheactivityofSODandCATwhiletheactivityofPODandthecontentofMDAreducedHKL_4treatmentsincreasedthebiomassofrootsandrhizomesoflicorice

13、6ThecontentsofIAA，GAandCTKsinleaves，stemsandrhizomesignificantincreasedbyHKL4treatmentwhileABAcontentsdecreasedHKL4treatmentssignificantlyincreasedthecontentsof1AA，GA4andCTKsinroots，whilethecontentsofABAinlatergrowthstag

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