大劑量NAP對新生大鼠缺氧缺血性腦損傷的保護作用及機制初探.pdf

1、山西醫(yī)科大學碩士學位論文大劑量NAP對新生大鼠缺氧缺血性腦損傷的保護作用及機制初探姓名：霍莉申請學位級別：碩士專業(yè)：兒科學指導教師：陰懷清20080505山西醫(yī)科大學碩士學位論文一一—————————————————————————————一ApproachtoLargeDoseNAP’sprotectionofneonatalratswlthhypoxicischemicbraindamageanditsmechanismsAbstr

2、actObjective：Toobservethecaspase一3andA[3142([3amyloid)expressmnVarlatlonof7daysratswitllhyp0Xicischemicbraindamage(nmD)，andtoapproachtheprotectlon研NAP(activitydependentneuroprotectiveprotein)，SOastosearchforanewposslblem

3、etIlodtotreatnewbornswithHIBDMethods：Atotalof78Wistarneonatalrats，cleanlinessstandard，femaleorm2Lle，withthebirthweightof16t020伊amwererandomlydividedinto(1)shamoperationgroup(2)HIBD露oup(3)NAPtreatmentgroupand(4)NAPprecond

4、itioninggroupTheratsmsh鋤operatlongroupwereoniycutofanickinthemiddleoftheircervicalpart，then咖ethelr1nclslons，theseratscoIIⅡ110ncervicalarterieswerenotbeenclampedandtheyhadnotundergoneHIBDAndtheotherratshadundergonehypoxic

5、ischemicbraindamage，theirrigh‘comm∞carotldart州wereligatedfor1hourthenbreathedwith8％oxygenand92％nitrogennm汜dgas，thehypo)【icischemicbraindamagemodelwerepreparedTheratsofNAPpreconditlomnggroupwereiniectedNAPintheirabdominal

6、cavities30minutesbeforeundergoingHIBDTheratsofNAPtre釉entgroupwereinjectedNAPaftertheyweretakenoutofmionecticboxIherats0tthesecond，thjrd鋤dfounllgroupwereobservedafterundergoingHIBDatthefirstdaythirddayfifuldayandseventhda

7、yrespectivelyTherewere6ratsineverytimep0Intslhetonnv撕ationofnervecellswereobservedbyHEstainingunderlightmicroscopeind1士士erent‘1mepoints，andthecaspase3andApl42expressionvariationwereeValuatedbYu。u11Ⅵ11妙。hiStochemiStrystai

8、ningoftheratsinshamoperationgroup，HIBDgroup，NAPtreatment筍oupandNAPpreconditioninggroupindifferenttimepointsResult：T11eratsofHIBDgroup，NAPtreatmentgroupandNAPpreconditioninggroupbehaveddisorderlyindifferentextentItishardt

9、ofindcaSpaSe3andA13t42Posl‘1Vecellinrats’palli哪0fsh鋤operationgroupunderlightmicroscopeCaspase一3andApI42poSitlVeceuswereincreasedobviouslyafterhypoxicischemiawhichCanbefoundafter1dayofhypoxicischemia，anduptothehighestonth