針刺對持續(xù)性高眼壓大鼠視網膜神經節(jié)細胞損害的干預研究.pdf

1、成都中醫(yī)藥大學碩士學位論文針刺對持續(xù)性高眼壓大鼠視網膜神經節(jié)細胞損害的干預研究姓名：黃珍珍申請學位級別：碩士專業(yè)：中醫(yī)五官科學（眼科學）指導教師：廖品正20060401成都中阪藥大學磺士學位瓴文ABSTRACTObjective：ToobservetheeffectofacBpunctureontheratmodelofchronic，moderatelyelevatedintraocularpressure(ioP)，andstudy

2、themechanismofitinitiallyMethods：Themodelofchronic，elevatedintraocularpressureratwasinducedbyAkriaTheratsweredividedinto3groupsrandomly：normalcontrolgroup，modelgroupandacupuncturetreatedgroupJingmingandTaichongpointsaret

3、hepointsofacupuncture，wereusedtotreattheratswithelevatedintraocularpressuretoobservetheeffectionOnmfERG，marphologieofretina。theapoptosisofretinalganglioncells(RGCs)bylabeleddextrampolymermethod(LDP)methodandmeasurethegan

4、glioneelllayerplyandnitricoxideinretinabynitratereductase《NO)method。Results：@Acupuncturecanpreventelevatingintraocularpressureeffectively。②Acupuncturecanimprovetheeyesightfunction：shorten那㈣P1wavetotalpeakfallowvolueandbu

5、iidupmfERGP1wavetotalfeedbackdensity。③AcupuncturecanrecedethethecontentofNOinretinaeffectively，consequentlyreduceitstoxicityeffecttoRGCs。④AcupuncturecanadjustandcontrolthegeneexpressioninRGCsofretina。Conclusion：aftercont

6、inuouselevatedintraocularpressureforlmonths。theexperimentaldiabeticratsshowedimmunohistochemJcatchangesofRGCs。Acupunctureo抽improvetheeyesightfunction。protectorli兩tentheapoptosisofRGCs。andpreventtheopticnervefromelevatedl