銀杏葉愈傷組織誘導、增殖及其生理特性的研究.pdf

1、華中師范大學碩士學位論文銀杏葉愈傷組織誘導、增殖及其生理特性的研究姓名：張君誠申請學位級別：碩士專業(yè)：植物學.植物細胞工程指導教師：周吉源19981201AbstractInthispaper，theeffectsofdifferenthormones、lightqualitiesandmediumsduringinductionandprolifirationofcallusfromGinkgobilobaLinVitrowerest

2、udiedAlsothechangesofphysiologicalandbiochemicalcharactersandaccumulationofsecondarymetabolite(flavonoids)werestudiedTheresultswereasfollows：Thecombinationsofdifferentkindsanddensityofhormonescouldmoreeffectivitylyinduce

3、theformationofcallusthanthesinglefactorTheeffeetsofMSmediumwithNAAand6一BAwerethebestWhitelightandgreenlightwereoptimalfortheinductionofcallusfromex—plantsandtheinductionfrequenciescouJdreach100％and95％Respee—tively，amongm

4、anykindsofmediums，MSand1／2MSwereoptlmslfortheinductionofcallusThegrowthcycleofculturedcellswasabout27days，andchangedintheformof“S”curveTheoptimalmediumsforproliferationofcalluswereMS、1／2MSand1／4MSWhitelightandredlightwer

5、eoptimalforproliferation0fcallusFromthebeginningtOthe3rddayofgrowthcycle，soluableproteincontentofculturedcellsreducedandthenbegantoincreasegraduallyandreachedapeakonthe21stday，afterthe21stday，itreducedslightlyagainPeroxi

6、daseactivityofculturedcellsappearedtwopeaksonthe3thdayandthe15thdayduringtheproliferationperiodPhenylatanineammonialgaseactivityofculturedcellsgottOtwopeaksonthe6thdayandthe18thdayTheculturedcellscouldproduceflavonoid，an

7、dthecontentrosetOthehighestonthe18thday，whichwas278％TheflavonoidcontentinculturedcellsinvitrowaslowerthanthatofleavesFinally，discussionsweregiventOtherelationbetweenhormonesandformationofcallus，theeffectsoflightqualities

8、duringformationandpro—liferationperiodofcallusFurthermore，therelationbetweenthemetabolismpathandtheflavonoidcontent0fculturedcellswerealsodiscussedKeyWords：Callus；Induction；Proliferation；Physiological；Characters：Floavoid